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61.
62.
冰草属植物组织培养再生体系的建立 总被引:10,自引:3,他引:10
以适宜西北地区种植的优质牧草冰草属(AgropyronGaertn)中的三个不同种———蒙古冰草新品系(A.mongolicumKeng)、航道冰草(A.cristatumcv.Fairway)、诺丹冰草(A.desertorumcv.Nordan)和一个种间杂种冰草———蒙农杂种冰草(A.cristatum×A.desertorumcv.Mengnong)为材料,分别以幼穗为外植体建立了冰草组织培养再生体系。诱导愈伤组织的培养基为改良MS+2,4 D2.0mg/L,分化培养基为MS(无附加成分),在1/2MS培养基上生根后得到完整小植株。结果表明在本试验条件下,不同长度的幼穗在培养时,其愈伤组织发生的部位及其增殖速度不同;4种材料愈伤组织诱导率和分化率无明显差异,均可有效诱导愈伤组织并分化成再生植株,再生植株的产生主要通过直接器官发生途径。 相似文献
63.
G. P. Chapman 《Euphytica》1995,81(2):121-129
Summary Techniques of inflorescence and spikelet culture have been used for three major cereals. In maize, the influence of hormones on sexuality has been demonstrated. For wheat and barley the influence of supplying carbohydrate and nitrogen on grain content has been explored. Although in vitro seed setting is less efficient than in vivo the resulting grains, from optimal treatments, are in almost all respects normal or nearly so. Culture media to achieve this are precisely defined.In reviewing this work a major concern has been to highlight an elegant system of enquiry but at the same time to indicate the remarkably small area of interest to which it has been applied. As a prelude therefore to a consideration of the technical achievements, the range of spikelet form, and more particularly, function are examined. The aim is to encourage the application of inflorescence and spikelet culture to a much wider range of topics than hitherto. 相似文献
64.
Summary Plants of tetraploid Festuca pratensis var. apennina (2n=28) collected from Switserland and Northern Italy produced few inflorescences when grown in the field and under glasshouse conditions. The effects of winter and spring treatments upon reproductive growth were investigated and it was concluded that exposure to low winter temperatures and the control of tiller density in the spring were two important factors controlling the production of inflorescences. A procedure for the production of seed is suggested which will allow breeders to obtain efficient multiplication of collected plant material. 相似文献
65.
N. W. Widstrom A. Butron B. Z. Guo D. M. Wilson M. E. Snook T. E. Cleveland R. E. Lynch 《European Journal of Agronomy》2003,19(4):563-572
Several resistance sources and resistance mechanisms to aflatoxin formation and corn earworm (Helicoverpa zea Boddie) damage to maize (Zea mays L.) have been identified. Based on this knowledge, experiments were initiated toward achievement of the following objectives: (1) to confirm earlier determinations on resistance traits of germplasm sources and to identify quantitative trait loci (QTL) associated with each of the traits, and (2) upon estimation of the degree of QTL effects on each trait, to generate a maize population, with chemical and physical resistance to Aspergillus spp. and ear-feeding insects, for inbred development. A 2-year field experiment to evaluate selected genotypes inoculated with A. flavus and infested with corn earworm revealed that significant variation exists among the genotypes for aflatoxin contamination and corn earworm damage. The protection of maize ears against aflatoxin contamination was primarily dependent on resistance to fungal infection and ear-feeding insects, and excellent husk coverage and tightness. A major QTL (p1) identified on chromosome 1S had effects of 54.0, 42.1, and 28.3% on the phenotypic variability for concentrations of silk maysin, 3′-methoxymaysin+apimaysin, and chlorogenic acid, respectively. Markers/QTLs for husk phenotypic traits and total aflatoxin concentrations have been determined, but more detailed mapping of these chromosomic regions will be necessary to locate precise markers/QTLs for husk traits and aflatoxin production. Realizing the complexity of the Aspergillus–aflatoxin-maize system and the factors affecting aflatoxin contamination, we are directing our program toward marker-assisted breeding to enhance or improve general genetic resistance to ear-feeding insects and invasion by Aspergillus spp. 相似文献
66.
The tomato floral system is distinguished from that of Arabidopsis and Antirrhinum in several ways. The shoot is a sympodium, the first flower of the inflorescence is apical and no bracts are formed. We discuss the possible function of genes affecting growth habit and inflorescence development and analyze in detail the unique developmental alterations caused by inhibition or ectopic expression of tomato-specific MADS-box genes. Preliminary analysis of transgenic tomato plants expressing an alien homeotic gene and several different chimeric genes of the MADS-box family is also reported. 相似文献
67.
孕穗期低温对玉米雌穗的伤害作用 总被引:8,自引:0,他引:8
在孕穗期(播种后65天),玉米植株经10℃低温处理2天和5天后,雌穗超氧物歧化酶(SOD)活性下降,膜脂过氧化作用增强,对生物膜系统产生直接伤害,电解质外渗率增大,电镜下可观察到线粒体、细胞质膜破坏、胞间连丝膨大变形,甚至断裂;粗糙内质网卷曲成空心圆状,进而引起生理代谢紊乱,使可溶性糖和游离氨基酸含量增加,淀粉、蛋白质含量下降,阻碍雌穗发育,减少穗粒数,引起减产。 相似文献
68.
Influence of race and post infection temperature on two components of partial resistance to wheat leaf rust in seedlings of wheat 总被引:2,自引:0,他引:2
C. J. M. Denissen 《Euphytica》1991,58(1):13-20
Summary Components of partial resistance, infection frequency and latency period, were determined in 71 winter and spring wheat genotypes in the seedling stage, after infection with three races of leaf rust (Felix 3B, Clement B and Betuwe 85C) at three different day/night temperature regimes (24/18°C, 18/12°C and 12/6°C). The genotypes were split into two groups and two separate experiments were carried out. Five genotypes, SVP 84039, Akabozu, Banco, BH 1146 and Orso, conferred a low infection frequency and a long latency period and Westphal 12A a long latency period, indicating a relatively high level of partial resistance. The correlation coefficient between infection frequency and latency period was low. Race-specificity was not found. There was a significant temperature effect on the latency period. In the second experiment the temperature x genotype interaction was significant. Temperature-response functions of transformed data demonstrated that the latency periods of four relatively resistant genotypes, Westphal 12A, Banco, BH 1146 and Orso and of Sarno and Mirela were most sensitive to temperature. The range between the genotypes with the longest and the shortest latency period was highest at 12°C. Therefore, low temperature regimes are preferred to distinguish differences in level of partial resistance. 相似文献
69.
Saicum Pintasen Chanakan Prom-u-thai Sansanee Jamjod Narit Yimyam Benjavan Rerkasem 《Euphytica》2007,157(1-2):27-34
Crown rust caused by Puccinia coronata Cda. f. sp. avenae Eriks., is a major fungal disease of cultivated oats (Avena sativa L.) in Tunisia. Six landrace oats (MtK2, JT5, BJ35, GT1, ZN3 and JT0) locally collected and previously screened for their relative oat crown rust resistance were used in this experiment. These
accessions were evaluated during a two cropping season in field plots under heavy natural oat crown rust infection. Assessment
criteria to crown rust reactions were AUDPC, latent period, uredinia size and number of uredinia per cm2 of infected leaf area. Principal components analysis showed that the variables were grouped in two components. These two
principal components explained 83.56% of the total variance. The projection of the point-cloud representing the populations
on the plan formed by the principal components ‘Fact 1’ (63.83%), in abscissa, and ‘Fact 2’ (19.73%) in ordinate, permitted
to distribute the accessions in several groups. Three landrace oats (MtK2, JT5, and JT0) showed the lowest value of all the components used. These landrace oats might be good sources of effective and durable resistance
to crown rust. 相似文献
70.
Sclerotinia sclerotiorum (Lib.) de Bary is a major limiting factor for sunflower (Helianthus annuus) production. This study was conducted to (1) evaluate a large number of current sunflower lines for their reaction to Sclerotinia, and (2) determine the optimum allocation of resources for their selection. A set of 85 inbred lines was screened under artificial leaf infection in three environments. The test revealed ample variation for all resistance traits in the current germplasm. A line selected from NDBLOS, an oilseed sunflower germplasm poo, and its derivatives were most resistant to Sclerotinia. Lines derived from interspecific crosses with the wild species Helianthus tuberosus and Helianthus argophyllus also showed satisfactory Sclerotinia resistance. The evaluation of five plants per plot in two replications and four environments appears to be a reasonable allocation of resources in order to optimize the selection response. 相似文献